Author

Taylor Carter

Document Type

Thesis

Date of Award

8-2022

School/College

College of Science, Engineering, and Technology (COSET)

Degree Name

MS in Biology

Committee Chairperson

Alamelu Sundaresan

Committee Member 1

Sodipe Ayodotun

Committee Member 2

Olufemi Shodimmu

Committee Member 3

Wilkerson Daryl

Keywords

ETAS, Heat Shock Protein Complex, Heat Shock Proteins, HSP70, Oxidative Stress

Abstract

Neurodegenerative disease in the CNS is usually a product of increased oxidative stress in the brain. In this study we tested the ability of an asparagus supplement ETAS to help reduce oxidative stress in the normal brains of Balb C mice. Oxidative stress pathways (Heat shock proteins) are usually cumulative in the damage they cause when disrupted. We treated normal Balb C mice with ETAS and had control groups with no ETAS supplementation in their regular diet. We then sacrificed the mice and conducted microarray studies to compare oxidative stress pathway genes. We also characterized the effects of regular oxidative cells in a mammalian cell model treated with alcohol to understand how oxidative stress impairs the functions of a normal neuronal cell. Results from both in vivo and in vitro experiments will be described in this study.

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