Document Type
Thesis
Date of Award
3-2025
School/College
College of Science, Engineering, and Technology (COSET)
Degree Name
MS in Biology
Committee Chairperson
Dr. Audrey Player
Committee Member 1
Dr. Mario Hollomon
Committee Member 2
Dr. Shodimu-Emmanuel Olufemi
Committee Member 3
Dr. Daniel Vrinceanu
Abstract
Triple-Negative Breast Cancer (TNBC) is known to be the most aggressive subtype of breast cancer, accounting for 15–20% of cases. In TNBC, three receptors commonly associated with breast cancer—estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptor 2 (HER2neu)—are under-expressed. Earlier studies identified MYBL1 as a major transcriptional activator overexpressed in TNBC, playing a critical role in tumor growth, differentiation and apoptosis. In previous research conducted by our lab, MYBL1 was knocked down in a TNBC cell line and the dataset saved for future use. In the current study, we re-analyzed the dataset and identified E2F4 and TP53 affected by the knockdown process, suggesting some relationship between MYBL1 and E2F4 and TP53 genes. E2F4, a transcriptional activator, and TP53, a tumor suppressor gene are being examined for their connection to MYBL1. We outline our re-analyses of the datasets using Affymetrix Transcriptome Analysis Console (TAC) software TAC4.0 software. Through this software, we can gain deeper insights into how the knockdown of MYBL1 affects gene expression. We summarize our selection of E2F4 and TP53 and show experimental analyses of the genes in non-tumor compared to TNBC cell lines.
Copyright
Copyright © for this work is retained by the author. Any documents and information presented are protected by copyright under US Copyright laws and are the property of the author. All Rights Reserved. For permission to use this content please contact the author or the Graduate School at Texas Southern University ([email protected]).
Recommended Citation
Butler, Emmanuel, "TAC4 Analyses Of MYBL1 Knockdown In MDA MB231 Cells And Identification And Validation of E2F4 And TP53 Differential Gene Expression" (2025). Theses (2016-Present). 98.
https://digitalscholarship.tsu.edu/theses/98