Document Type

Thesis

Date of Award

10-2024

School/College

College of Science, Engineering, and Technology (COSET)

Degree Name

MS in Biology

Committee Chairperson

Dr. Audrey Player

Committee Member 1

Dr. Ayodotun Sodipe

Committee Member 2

Dr. Shodimu-Emmanuel Olufemi

Committee Member 3

Dr. Tuan Phan

Abstract

Accounting for 15-20% of all breast cancers, triple-negative breast cancer (TNBC), is the most aggressive subtype, with the worst prognosis. In previous studies, the MYBL1 gene, which is associated with several events that are key to tumor progression, has been identified as a powerful transcriptional activator that is overexpressed in TNBC. In earlier research, our lab knocked down MYBL1 expression in a TNBC cell line and identified a subset of genes impacted by the knockdown process. MYBL1 gene is located on the chromosome 8q locus. In addition to MYBL1 we identified 2 genes, VXN and CCNE2, also located at the 8q loci. This is of interest because the 8q loci is known to be a hotspot for gains and amplification mutations in genes associated with TNBC. We intend to further characterize the relationship between MYBL1, VXN, and CCNE2 using an analysis of both their RNA and protein expression patterns in triple negative tumor and non-tumor cell lines, as well as in patient samples. If these data validate, we will later examine the possibility that the genes are contributing to tumor progression in TNBC. Data presented in this study show that MYBL1, CCNE2 and VXN genes, all on chromosomal 8q loci demonstrate differential expression in TNBC cell lines and patient samples. Our data suggest MYBL1 and the genes identified in our studies can be considered for their role in TNBC oncogenic progression.

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