Document Type
Thesis
Date of Award
5-2022
School/College
College of Science, Engineering, and Technology (COSET)
Degree Name
MS in Biology
Committee Chairperson
Audrey Player
Committee Member 1
Erica Cassimere
Committee Member 2
Mario Hollomon
Committee Member 3
Tuan Phan
Keywords
ADRM1, Cancer, MYBL1, MYBL2, Triple Negative Cancer, UBXN8
Abstract
A previous study conducted in our laboratory demonstrated V-Myb Avian Myeloblast Viral Oncogene Homolog Like 1 (MYBL1) gene over-expression in triple negative breast cancer (TNBC) compared to normal, some luminal, and a subpopulation of other TNBC. The MYBL1 gene belongs to the Avian myeloblastosis virus (MYB) family and is classified as proto-oncogene that functions as a strong transcription factor. The MYBL1 gene is related to cancer progression which involves dysregulation of cell cycle signaling, apoptosis and differentiation processes. A primary goal of our laboratory is to further characterize MYBL1 gene expression in TNBC samples. To achieve this goal, we performed a knockdown study to identify genes that co-operate with MYBL1 to affect the phenotype of TNBC. The MDA MB231 TNBC cells were ransduced with a short hairpin ribonucleic acid (shRNA) lentiviral knockdown of the MYBL1 gene. When MYBL1 was knocked down, MYBL2 and Adhesion Regulating Molecule 1 (ADRM1) genes were down regulated and UBX Domain Protein 8 (UBXN8) gene was unregulated. Since MYBL2, UBXN8 and ADRM1 were affected by MYBL1 knockdown, for the current study, we compared the gene expression patterns of MYBL2, UBXN8 and ADRM1 to that of MYBL1 using different methods. Two approaches are utilized to achieve our goal. For approach 1 we utilized polymerase chain reaction and immunohistochemistry to assess RNA and protein expression levels, respectively. For the second approach, we analyzed MYBL1, MYBL2, UBXN8 and ADRM1 transcript levels in TNBC patient samples etrieved from Gene Expression Omnibus Results from this project should assist in our understanding of MYBL1 in TNBC.
Copyright
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Recommended Citation
Nsende, Esther Jensinne, "Experimental Validation of Gene Expression of MYBL1, MYBL2, UBXN8, and ADRM1 Genes in Triple Negative Breast Cancer Cell Lines" (2022). Theses (2016-Present). 13.
https://digitalscholarship.tsu.edu/theses/13