Effect of arsenic acid withdrawal on hepatotoxicity and disruption of erythrocyte antioxidant defense system
Document Type
Article
Publication Date
1-1-2017
Abstract
We investigated the effects of withdrawal from Sodium arsenite (NaAsO2) on the hepatic and antioxidant defense system in male Wistar rats using a before and after toxicant design. Rats were orally gavaged daily with varying doses of NaAsO2 for a period of 4 weeks. One half of the population was sacrificed and the remaining half had the toxicant withdrawn for another further 4 weeks. Biochemical and immunohistochemical techniques were used to assess the impact of withdrawal on the erythrocyte and hepatic systems. Exposure of Wistar rats to NaASO2 led to a significant (p < 0.05) increase in hepatic and erythrocyte markers of oxidative stress (malondialdehyde, thiol contents and hydrogen peroxide generation). Concurrently, there was a significant (p < 0.05) increase in hepatic and erythrocyte antioxidant enzymes (glutathione-S-transferase, glutathione peroxidase and superoxide dismutase) following exposure. Withdrawal from NaAsO2 exposure led to a decline in both erythrocyte and hepatic markers of oxidative stress and together with a significant improvement in antioxidant defense system. Histopathology and immunohistochemistry revealed varying degrees of recovery in hepatocyte ultrastructure alongside increased expression of the pro-survival protein Kinase B (Akt/PKB) after 4 weeks of NaAsO2 withdrawal. Conclusively, withdrawal from exposure led to a partial recovery from oxidative stress-mediated hepatotoxicity and derangements in erythrocyte antioxidant system through Akt/PKB pathway.
Recommended Citation
Oyagbemi, A. A.; Omobowale, T. O.; Asenuga, E. R.; Afolabi, J. M.; Adejumobi, O. A.; Adedapo, A. A.; and Yakubu, M. A., "Effect of arsenic acid withdrawal on hepatotoxicity and disruption of erythrocyte antioxidant defense system" (2017). Faculty Publications. 210.
https://digitalscholarship.tsu.edu/facpubs/210