Document Type


Date of Award



College of Science, Engineering, and Technology (COSET)

Degree Name

MS in Biology

First Advisor

Dr. Fawzia Abdel-Rahman


Azote (AZO) dyes are a large class of synthetic organic dyes that contains in its structure an azo group, R-N=N-R', and make up most of the commercial dyes used in clothes and some in food. Some of these dyes have been found to cause carcinogenesis in different specimens. Caenorhabditis elegans is used to test the toxicity of several azo dyes at 50􁪽g/mL (50 ppm) and 20 ug/ml. (20 ppm) concentrations through bioassay. The brood size and life span are also determined placing the C. elegans onto Nematode Growth Media (NGM) agar that contains the concentrations of the highly toxic azo dyes used in the experiment. Four azo dyes (4-Aminoazobenzene, Disperse Blue 124, Disperse Orange 3, and Disperse Blue 1) were selected to do further studies based on their high mortality rate (45% or higher). It was determined that of the four containing the highest mortality rate, azo dyes 4-Aminoazobenzene and Disperse Orange 3 one hundred percent (100%) mortality at the 50􁪽g/mL concentration and over 45% at the 20 ug/ml. concentration. On the NGM, azo dye 4-Aminoazobenzene showed that the hermaphroditic nematode had no reproduction at the 50􁪽g/mL concentration and very 1 2 minimal with the 20 ug/ml, concentration, Disperse Blue 1 showed a increase in reproduction, and Disperse Orange 3 and Disperse Blue 124 showed the same brood size as the control. Also, an interesting observation made was that there were different morphological or phenotypic effects on C. elegans exposed to the four azo dyes selected. It is suggested that the four azo dyes are looked at further in the Caenorhabditis elegans to determine their LCso and the genetic defects caused by each dye