Document Type


Date of Award



College of Science, Engineering, and Technology (COSET)

Degree Name

MS in Biology


Bacteria require optimal growth conditions in order to thrive. However, stress factors such as cold and oxidation can attribute to the pathogenecity of a species. Ribonuclease E ( RNase E) is a component of the multicomplex protein, the degradosome, which is inclusive of: enolase (a glycolytic enzyme), and RhlB helicase, and 3' -5' exoribonuclease polynucleotide phosphorylase (PNPase). The microbiological model organism, E. coli, is attributed to first indicating the presence of microdomain PNPase in the degradosome. However, the species of interest in this study is Yersinia pseudotuberculosis (YPT) due to its affinity to proliferate and sustain in said abiotic stress environments unlike its enterobacteriaceae counterpart. YPT exposed to oxidative stress need degradosome assembly therefore it is degradosome-dependent. Conversely, the cold growth mechanism of PNPase in YPT is degradosome-independent.